Disease:


  • OMIM ID: 601834 . 601834
  • OMIM diseaseName:
  • OMIM diseaseClinical_Synopsis:
  • OMIM diseaseText: For general information about chemokines and their receptors, see CCR1 (601159); see also CCR6 (601835). CLONING Zaballos et al. (1996) used degenerate PCR to isolate genes encoding putative chemokine receptors. One such gene, which they termed CKRL1, encodes a polypeptide of 355 amino acids with greatest homology to the family of beta-chemokine-binding receptors. Northern blot analysis revealed that CKRL1 is preferentially expressed as a 4.3-kb transcript in thymus. Independently, Napolitano et al. (1996) and Tiffany et al. (1997) cloned a cDNA corresponding to CCR8. Napolitano et al. (1996) detected an approximately 4.0-kb CCR8 transcript at high levels in spleen and thymus as well as at barely detectable levels in peripheral blood leukocytes. They also found abundant expression in NK and T cell lines. Tiffany et al. (1997) found expression of a 4.6-kb CCR8 transcript in monocytes but not in peripheral blood lymphocytes or neutrophils. GENE STRUCTURE By genomic clone analysis, Napolitano et al. (1996) determined that CCR8, which they called TER1, contains only 1 exon. MAPPING Zaballos et al. (1996) used analysis of somatic cell hybrids to map the CKRL1 gene to human chromosome 3. By FISH, Napolitano et al. (1996) and Tiffany et al. (1997) refined the localization of CCR8 to 3p21-p22, clustered with other chemokine receptor genes. ANIMAL MODEL In mice with a targeted deletion of the Ccr8 gene, Chensue et al. (2001) observed impaired Th2 cytokine production and eosinophil recruitment in response to Th2-sensitizing antigens such as Schistosoma mansoni soluble egg antigen-induced granuloma formation and ovalbumin- and cockroach antigen-induced allergic airway inflammation. In contrast, Th1 responses to purified protein derivative of Mycobacterium bovis were completely intact. RT-PCR analysis readily detected CCR3 (601268) but not CCR8 on wildtype eosinophils. RT-PCR and ELISA analysis revealed that type I cytokine responses were normal, whereas type II cytokine responses in lung and regional lymph nodes were markedly reduced, particularly the levels of IL5 (147850) and IL13 (147683); IL4 (147780) levels were not affected.
  • OMIM diseaseSee_Also:
  • OMIM diseaseAllelic_Variants:
  • OMIM diseaseCreation_Date: Jennifer P. Macke: 5/29/1997
  • OMIM diseaseEdit_History_Data: mgross: 09/26/2002 mgross: 10/8/2001 carol: 6/6/2000 alopez: 7/27/1999 mark: 1/13/1998 mark: 1/12/1998 alopez: 11/18/1997 mark: 7/3/1997 alopez: 6/24/1997 alopez: 6/10/1997 alopez: 6/5/1997
  • OMIM diseaseContributors: Paul J. Converse - updated: 10/8/2001 Paul J. Converse - updated: 6/6/2000
  • OMIM diseaseReference: 1. Chensue, S. W.; Lukacs, N. W.; Yang, T.-Y.; Shang, X.; Frait, K. A.; Kunkel, S. L.; Kung, T.; Wiekowski, M. T.; Hedrick, J. A.; Cook, D. N.; Zingoni, A.; Narula, S. K.; Zlotnik, A.; Barrat, F. J.; O'Garra, A.; Napolitano, M.; Lira, S. A.: Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J. Exp. Med. 193: 573-584, 2001. 2. Napolitano, M.; Zingoni, A.; Bernardini, G.; Spinetti, G.; Nista, A.; Storlazzi, C. T.; Rocchi, M.; Santoni, A.: Molecular cloning of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J. Immun. 157: 2759-2763, 1996. 3. Tiffany, H. L.; Lautens, L. L.; Gao, J. L.; Pease, J.; Locati, M.; Combadiere, C.; Modi, W.; Bonner, T. I.; Murphy, P. M.: Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309. J. Exp. Med. 186: 165-170, 1997. 4. Zaballos, A.; Varona, R.; Gutierrez, J.; Lind, P.; Marquez, G. : Molecular cloning and RNA expression of two new human chemokine receptor-like genes. Biochem. Biophys. Res. Commun. 227: 846-853, 1996. Note: Erratum: Biochem. Biophys. Res. Commun. 231: 519 only, 1997.