- OMIM ID: 601834
- OMIM diseaseName:
- OMIM diseaseClinical_Synopsis:
- OMIM diseaseText: For general information about chemokines and their receptors, see CCR1
(601159); see also CCR6 (601835).
Zaballos et al. (1996) used degenerate PCR to isolate genes encoding
putative chemokine receptors. One such gene, which they termed CKRL1,
encodes a polypeptide of 355 amino acids with greatest homology to the
family of beta-chemokine-binding receptors. Northern blot analysis
revealed that CKRL1 is preferentially expressed as a 4.3-kb transcript
in thymus. Independently, Napolitano et al. (1996) and Tiffany et al.
(1997) cloned a cDNA corresponding to CCR8. Napolitano et al. (1996)
detected an approximately 4.0-kb CCR8 transcript at high levels in
spleen and thymus as well as at barely detectable levels in peripheral
blood leukocytes. They also found abundant expression in NK and T cell
lines. Tiffany et al. (1997) found expression of a 4.6-kb CCR8
transcript in monocytes but not in peripheral blood lymphocytes or
By genomic clone analysis, Napolitano et al. (1996) determined that
CCR8, which they called TER1, contains only 1 exon.
Zaballos et al. (1996) used analysis of somatic cell hybrids to map the
CKRL1 gene to human chromosome 3. By FISH, Napolitano et al. (1996) and
Tiffany et al. (1997) refined the localization of CCR8 to 3p21-p22,
clustered with other chemokine receptor genes.
In mice with a targeted deletion of the Ccr8 gene, Chensue et al. (2001)
observed impaired Th2 cytokine production and eosinophil recruitment in
response to Th2-sensitizing antigens such as Schistosoma mansoni soluble
egg antigen-induced granuloma formation and ovalbumin- and cockroach
antigen-induced allergic airway inflammation. In contrast, Th1 responses
to purified protein derivative of Mycobacterium bovis were completely
intact. RT-PCR analysis readily detected CCR3 (601268) but not CCR8 on
wildtype eosinophils. RT-PCR and ELISA analysis revealed that type I
cytokine responses were normal, whereas type II cytokine responses in
lung and regional lymph nodes were markedly reduced, particularly the
levels of IL5 (147850) and IL13 (147683); IL4 (147780) levels were not
- OMIM diseaseSee_Also:
- OMIM diseaseAllelic_Variants:
- OMIM diseaseCreation_Date: Jennifer P. Macke: 5/29/1997
- OMIM diseaseEdit_History_Data: mgross: 09/26/2002
- OMIM diseaseContributors: Paul J. Converse - updated: 10/8/2001
Paul J. Converse - updated: 6/6/2000
- OMIM diseaseReference: 1. Chensue, S. W.; Lukacs, N. W.; Yang, T.-Y.; Shang, X.; Frait, K.
A.; Kunkel, S. L.; Kung, T.; Wiekowski, M. T.; Hedrick, J. A.; Cook,
D. N.; Zingoni, A.; Narula, S. K.; Zlotnik, A.; Barrat, F. J.; O'Garra,
A.; Napolitano, M.; Lira, S. A.: Aberrant in vivo T helper type 2
cell response and impaired eosinophil recruitment in CC chemokine
receptor 8 knockout mice. J. Exp. Med. 193: 573-584, 2001.
2. Napolitano, M.; Zingoni, A.; Bernardini, G.; Spinetti, G.; Nista,
A.; Storlazzi, C. T.; Rocchi, M.; Santoni, A.: Molecular cloning
of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J.
Immun. 157: 2759-2763, 1996.
3. Tiffany, H. L.; Lautens, L. L.; Gao, J. L.; Pease, J.; Locati,
M.; Combadiere, C.; Modi, W.; Bonner, T. I.; Murphy, P. M.: Identification
of CCR8: a human monocyte and thymus receptor for the CC chemokine
I-309. J. Exp. Med. 186: 165-170, 1997.
4. Zaballos, A.; Varona, R.; Gutierrez, J.; Lind, P.; Marquez, G.
: Molecular cloning and RNA expression of two new human chemokine
receptor-like genes. Biochem. Biophys. Res. Commun. 227: 846-853,
1996. Note: Erratum: Biochem. Biophys. Res. Commun. 231: 519 only,